Journal: Respiratory Research

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10

doi: 10.1186/s12931-026-03640-3

Figure Lengend Snippet: Targeting B cells improves overall lung function. A Gene Ontology term enrichment analysis of upregulated genes in anti-CD20-treated emphysema mice compared to untreated emphysema mice. B Volcano plot of differentially expressed genes in B1 cells of the anti-CD20 group versus the Emphysema group, plotting the log2-transformed fold change (log2FC) against the statistical significance (-log10 of the q -value). Significantly up- or downregulated genes ( q -value < 0.05, log2FC > 0.1 or log2FC < − 0.1, respectively) are marked in red or blue and selected upregulated and downregulated genes are labeled. C GSEA showing the upregulation of electron transport chain, respirasome and TGF-beta signaling pathway in emphysema and anti-CD20-treated emphysema mice. D Lung function analysis of enhanced pause (Penh), peak expiratory flow resistance (Rpef) and expiratory time (Te) value of control mice, emphysema mice and anti-CD20 antibody-treated emphysema mice. Data were presented as mean ± SEM (by unpaired Student’s t test and one-way ANOVA). ADAM10i: ADAM10 inhibitor-treated emphysema mice

Article Snippet: The following five experimental groups were established: (1) T cells + control; (2) T cells + B2 cells; (3) T cells + B1 cells; (4) T cells + B1 cells + 10 μM ADAM10 inhibitorGI254023X (HY-19956, MedChemExpress, USA); (5) T cells + B1 cells + 50 nMNotch inhibitorBMS-906,024 (HY-15670, MedChemExpress, USA).Cells were treated by ADAM10 inhibitorat 10 μM [ ] and Notch inhibitorat 50nM [ ].

Techniques: Transformation Assay, Labeling, Control

Journal: Respiratory Research

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10

doi: 10.1186/s12931-026-03640-3

Figure Lengend Snippet: ADAM10 upregulation in B1 cells promotes emphysema pathogenesis. A Violin plot showing the expression level of Adam10 in different B cell subsets of control and emphysema mice. B Violin plot showing the significant enrichment of the Notch signaling pathway gene set in B1 cells of emphysema mice compared with the control. C Violin plots displaying the expression levels of Notch signaling target genes ( Dtx1 , Hes1 , Hes5 ) in different B subsets from control and emphysema mice. D Immunofluorescence staining for IgM (red), ADAM10 (green) and DAPI (blue) in the lungs of control mice and emphysema mice. Scale bars: 25 μm (low-power view), 10 μm (high-power view). E Number of IgM + ADAM10 + cells / mm 2 in the lungs. F Flow cytometric analysis of ADAM10 + cells within B1 cells in lungs. Data were presented as mean ± SEM (by unpaired Student’s t test)

Article Snippet: The following five experimental groups were established: (1) T cells + control; (2) T cells + B2 cells; (3) T cells + B1 cells; (4) T cells + B1 cells + 10 μM ADAM10 inhibitorGI254023X (HY-19956, MedChemExpress, USA); (5) T cells + B1 cells + 50 nMNotch inhibitorBMS-906,024 (HY-15670, MedChemExpress, USA).Cells were treated by ADAM10 inhibitorat 10 μM [ ] and Notch inhibitorat 50nM [ ].

Techniques: Expressing, Control, Immunofluorescence, Staining

Journal: Respiratory Research

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10

doi: 10.1186/s12931-026-03640-3

Figure Lengend Snippet: Inhibition of ADAM10 alleviates emphysema and TLS maintenance. A Representative images of HE staining in the lungs (airway and parenchyma) of control mice, emphysema mice and ADAM10 inhibitor-treated emphysema mice. Scale bars, 200 μm. B - C Inflammation score ( B ) and MLI ( C ) in the lungs. D Representative images of H&E staining (Scale bars, 200 μm) and immunofluorescence for CD3 (red), CD20 (green) and DAPI (blue) (Scale bars, 25 μm) in the lungs of control mice, emphysema mice and ADAM10 inhibitor-treated emphysema mice. E Number of TLS / mm 2 in the lungs. F Lung function analysis of Penh, Rpef and Te value of mice ( G ) Hb concentration in blood of mice. H qPCR quantification of Hes1 , Hes5 and Hey1 expression in the lungs. Data were presented as mean ± SEM (by one-way ANOVA). ADAM10i: ADAM10 inhibitor-treated emphysema mice

Article Snippet: The following five experimental groups were established: (1) T cells + control; (2) T cells + B2 cells; (3) T cells + B1 cells; (4) T cells + B1 cells + 10 μM ADAM10 inhibitorGI254023X (HY-19956, MedChemExpress, USA); (5) T cells + B1 cells + 50 nMNotch inhibitorBMS-906,024 (HY-15670, MedChemExpress, USA).Cells were treated by ADAM10 inhibitorat 10 μM [ ] and Notch inhibitorat 50nM [ ].

Techniques: Inhibition, Staining, Control, Immunofluorescence, Concentration Assay, Expressing

Journal: Respiratory Research

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10

doi: 10.1186/s12931-026-03640-3

Figure Lengend Snippet: ADAM10 in B1 cells is associated with T cells activation. A Flow cytometric analysis of B1a cells (CD19 + CD23 − IgM + CD43 + CD5 + ) and B1b cells (CD19 + CD23 − IgM + CD43 + CD5 − ) in lungs of control mice, emphysema mice and ADAM10 inhibitor-treated emphysema mice. B - C Flow cytometric analysis of ADAM10 + cells within B1 cells in lungs. D - E Flow cytometric analysis of ICOS + cells within CD4 + T cells in lungs. F ELISA analysis of serum anti-PCNA and ARPA levels. G Flow cytometric analysis of percentage of proliferating cells in CD4 + T cells and B cells ( n = 6). H Flow cytometric analysis of ICOS+ cells within CD4 + T cells in different groups of co-cultured cells in vitro ( n = 3). Data were presented as mean ± SEM (by one-way ANOVA). ADAM10i: ADAM10 inhibitor-treated emphysema mice

Article Snippet: The following five experimental groups were established: (1) T cells + control; (2) T cells + B2 cells; (3) T cells + B1 cells; (4) T cells + B1 cells + 10 μM ADAM10 inhibitorGI254023X (HY-19956, MedChemExpress, USA); (5) T cells + B1 cells + 50 nMNotch inhibitorBMS-906,024 (HY-15670, MedChemExpress, USA).Cells were treated by ADAM10 inhibitorat 10 μM [ ] and Notch inhibitorat 50nM [ ].

Techniques: Activation Assay, Control, Enzyme-linked Immunosorbent Assay, Cell Culture, In Vitro

Journal: Respiratory Research

Article Title: B1 cells drive pulmonary emphysema progression through ADAM10

doi: 10.1186/s12931-026-03640-3

Figure Lengend Snippet: ADAM10 expression in B1 cells from patients with COPD. A UMAP plot showing the identified cell types of lung B cells. B Dot plot showing the expression of the marker genes in each B cell subsets. C Relative percentages of different B cell subsets in lungs of control and patients with COPD. D Violin plot showing gene expression level in lungs of control and patients with COPD. E Enriched Gene Ontology functions of upregulated genes in the B1 cell cluster

Article Snippet: The following five experimental groups were established: (1) T cells + control; (2) T cells + B2 cells; (3) T cells + B1 cells; (4) T cells + B1 cells + 10 μM ADAM10 inhibitorGI254023X (HY-19956, MedChemExpress, USA); (5) T cells + B1 cells + 50 nMNotch inhibitorBMS-906,024 (HY-15670, MedChemExpress, USA).Cells were treated by ADAM10 inhibitorat 10 μM [ ] and Notch inhibitorat 50nM [ ].

Techniques: Expressing, Marker, Control, Gene Expression